DNA Fabrication

Sequence files discussed in these slides available on bcourses as “DNA Fabrication Examples.zip”

YouTube playlist:
https://www.youtube.com/watch?v=_XpQgGAO0lw&list=PL2aPXzks-TgPX7HU4pogNUSBN9OK2PhBK

Different strategies at different scales

• Basic Cloning
• Ad hoc Assembly
• Gene Synthesis
• BioBrick Assembly
• Homology-based Assembly
• Golden Gate Assembly