BioBrick
Standard Assembly

Building up larger DNAs from standardized intermediates (Parts)

Biobricks

Biobricks

EcoRI         XbaI       partA            SpeI         PstI
GAATTCGCGGCCGCATCTAGAGTCCCTAGATACTGAGCACTACTAGTAGCGGCCGCTGCAG
CTTAAGCGCCGGCGTAGATCTCAGGGATCTATGACTCGTGATGATCATCGCCGGCGACGTC
EcoRI          XbaI      partB         SpeI         PstI
GAATTCGCGGCCGCATCTAGATCTCCCTTCGGGAAGCGTACTAGTAGCGGCCGCTGCAG
CTTAAGCGCCGGCGTAGATCTAGAGGGAAGCCCTTCGCATGATCATCGCCGGCGACGTC
↓ Digest
EcoRI         XbaI       partA            SpeI  XbaI      partB         SpeI         PstI
 AATTCGCGGCCGCATCTAGAGTCCCTAGATACTGAGCACTA       CTAGATCTCCCTTCGGGAAGCGTACTAGTAGCGGCCGCTGCAG
     GCGCCGGCGTAGATCTCAGGGATCTATGACTCGTGATGATC       TAGAGGGAAGCCCTTCGCATGATCATCGCCGGCGACGTC
↓ Ligate
EcoRI         XbaI       partA           (scar)    partB         SpeI         PstI
 AATTCGCGGCCGCATCTAGAGTCCCTAGATACTGAGCACTACTAGATCTCCCTTCGGGAAGCGTACTAGTAGCGGCCGCTGCAG
     GCGCCGGCGTAGATCTCAGGGATCTATGACTCGTGATGATCTAGAGGGAAGCCCTTCGCATGATCATCGCCGGCGACGTC

Biobricks

Optional slides from YouTube:
https://www.youtube.com/watch?v=F1mXoy1-Vr0&list=PL2aPXzks-TgPX7HU4pogNUSBN9OK2PhBK&index=4

Still popular in iGEM program, but slow relative to other assembly methods

Often requires gel purification, which can be tricky, and super hard for lower-copy plasmids