Run a gel

SYBR Green / Safe

SYBR Green
(maybe not a carcinogen?)….wear gloves

Ethidium Bromide
(carcinogen)

An Invitrogen Safe Imager on the bench: a black box whose top is a deep orange filter screen, lit from underneath by a strip of blue light along the front edge. A pair of orange safety goggles is lying on the filter. A gel rig sitting on top of a Bio-Rad PowerPac 1000 power supply. The clear acrylic tank has a comb in place at one end and two yellow hazard triangles on its wall; a red lead and a black lead run from its lid down to the power supply's terminals.

Blue light imager (not UV)….but wear orange goggles

Run the gel

  1. Add 8 uL loading dye and 3 uL PCR product to a new PCR tube
  2. Cut off a slab of pre-poured 1% TAE Agarose Gel
  3. Load 9uL samples and an additional lane of 1 kb ladder (NEB)
  4. Run the gel at 175 volts for around 10 min
  5. Take a picture, describe in a Github issue, summarize the results

Loading dye contains:

  • Bromphenol Blue and Xylene Cylanol blue dyes
  • EDTA (to chelate any Mg ions and prevent reactions)
  • Glycerol (makes it dense)
  • SYBR Green (none in the gel or running buffer)

TAE contains:

  • Tris (buffer at neutral pH)
  • Acetate (counterion to TRIS)
  • EDTA (chelate Mg)