Zymo Cleanup

Zymo Research: DNA Clean & Concentrator

The Zymo Research DNA Clean and Concentrator-5 kit as it is sold: a yellow box with the Zymo Research logo, flanked by a bottle of DNA Wash Buffer and a bottle of DNA Binding Buffer, a small vial of elution buffer, and a row of six small clear spin columns lying in front.

Qiagen: QIAquick PCR Purification Kit Print

The Qiagen QIAquick PCR Purification Kit (250) as it is sold: a blue box, two large bottles labelled Buffer PBI binding buffer and Buffer PE wash buffer, a small bottle of Buffer EB elution buffer, and several purple-topped spin columns lying beside it.

Zymo Cleanup Chemistry

DNA Purification Columns

Adapted from takarabio.com

Zymo Cleanup

The Regular Zymo Cleanup cheatsheet. It removes the polymerase, dNTPs, buffer and most of the oligonucleotides from a PCR reaction, and the buffer and restriction enzymes from a digest. Nine steps: add 180 microlitres of Zymo ADB buffer to the reaction; transfer into the Zymo column; spin at full speed 15 seconds and discard the waste; add 200 microlitres of PE Buffer; spin 15 seconds, discard; add 200 microlitres of PE Buffer again; spin 15 seconds, discard; spin 90 seconds to dry the sample, discard; elute with Elution Buffer into a fresh 1.5 millilitre Eppendorf tube, adding the volume on your protocol sheet down the middle of the column without letting it stick to the sides, then spin 45 seconds.

There are also variant protocols for small fragments (<200 bp long) and for DNA embedded in agarose gel