Genome
Editing

Example files available on bcourses as “Genome Editing Examples.zip”

YouTube playlist:
https://www.youtube.com/watch?v=vfmuDvEgD28&list=PL2aPXzks-TgOtngzKdocz4P50TCncRyPy

• Phage Integration
• Site-Specific Recombinases
• Homologous Recombination
• CRISPR
• Conjugation
• Transposons
• Phage Transduction

Why change the genome?

• Most applications require both the addition of functions and removal of existing genes (ex, knocking out a branch of biosynthesis for small molecule production)
• For larger systems, it is difficult to put large numbers of genes into plasmids
• Get better genetic stability (plasmids can get lost somewhat easily)
• Many plasmids in one cell is usually a little toxic and unstable
• To preserve local context
• Best way to get stable “single-copy” number