Picking colonies
- Pick well-rounded, isolated colonies representing distinguishable phenotypes
- For pP6 it’s the greenest things you find, or other interesting phenotypes
- Usually 2 to 4 colonies
- Photograph the plate for records
- Fill a 15 mL conical with 4 mL of 2YT containing antibiotic (carb)
- Stab or scoop with a sterile tip or toothpick, then drop the utensil in
- Incubate with shaking overnight
Step by step: Colony Picking tutorial ·
bench cheatsheet
Seven things, and the first two are about choosing rather than doing. Everything after that is mechanical.
Seven points on picking colonies, arriving one at a time. The first is on the slide already: pick well-rounded, isolated colonies representing distinguishable phenotypes.
