Directed Evolution

YouTube playlist:
youtube.com/watch?v=1wWyTrUroGM&list=PL2aPXzks-TgOOPfxneYJ3pBwTlqzv9Ezn

Protein Engineering Overview

Rational design (look at the crystal structure or multiple-sequence alignments)
Computational algorithm-based design (example: Rosetta)
Modular design (fusing domains together)
Library strategies (directed evolution and combinatorial methods)

Translational Fusions Tutorial(optional)

A translational fusion drawn as a chain of interlocking coloured blocks: V-heavy in dark blue, a grey linker, V-light in red, a second blue linker, and pIII in green. Beneath, the same five blocks are listed one per row against the part name each stands for: V_H, linker1, V_L, linker2 and p3.

openwetware.org/wiki/Arking:JCAOligoTutoria20

Structural Biology Tutorial(optional)

Introduction to Structural Biology and Part Engineering
DeepView Basics
Tour of Structural Biology

A protein structure rendered in DeepView against a black background: the backbone drawn as a white ribbon mesh, side chains as thin coloured lines, and a dotted surface around the whole molecule.