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Oligo Finder Tool

This tool allows you to upload a directory of oligonucleotide files and enter a DNA sequence to identify matching oligos that will anneal to the template.





Instructions

  1. Click Choose Folder to upload .txt or .tsv files containing your oligo list.
  2. You may select a single file, a folder containing files, or a folder with nested folders containing files.
  3. Each file should contain lines with name<TAB>sequence, where:
  4. name includes only letters, numbers, underscores, dashes, or dots.
  5. sequence includes valid IUPAC DNA codes (A, T, C, G, N, R, Y, etc.).
  6. Enter a target DNA sequence in the text box.
  7. Click Analyze to find oligos with 3′ matches that anneal to your sequence.

  8. Use Clear to reset the stored oligo data.

  9. ⚠️ This tool only checks for forward strand matches. To check for reverse strand annealing, input the reverse complement of your target sequence.

This tool runs entirely in your browser and works offline. No data is sent over the internet.
Extracted oligo data is saved in your browser's storage and will persist across sessions unless you clear it using the Clear button.
⚠️ This tool only searches for oligos that anneal to the forward strand. To check the reverse strand, input the reverse complement of your target sequence.