Bench Cheatsheets

A one-page card for each wetlab protocol. Copies are at the bench — these are the same cards, here so you can look at one before lab, or from anywhere.

They are quick-start cards, not tutorials. They assume you have read the tutorial and want the volumes, the order, and the things that go wrong quietly.

The whole set

⬇ All nine in one file — 9 pages, in experiment order. Useful if a bench copy has gone missing and needs reprinting.

Individual sheets

Cheatsheet	What it covers	Tutorial
PrimeSTAR PCR	50 µL reaction, master mix for 5+	Polymerase Chain Reaction
Analytical Gel	Sample prep, loading, running, imaging	Gel Electrophoresis
Zymo Cleanup	Column cleanup of a PCR or digest	Zymo Cleanup
Golden Gate Assembly	10 µL reaction, GG1 program	Assembly
KCM Heat-Shock Transformation	Block timings, rescue, plating	Transformation
Picking Colonies	Tubes or a block, which colonies	Colony Picking
Qiagen Miniprep	Alkaline lysis and column	Miniprep
Cycle Sequencing	13 µL submission, dGTP protocol	Cycle Sequencing
Tecan M Nano: Fluorescence + OD600	Start-up order, read settings, saving	Measuring Fluorescence

If a sheet looks wrong

Each card is generated from the same protocol module the Protocol Builder uses, so a card and the protocol it comes from cannot disagree — fixing one fixes both.

So do not edit the PDF, and do not edit the HTML beside it; both are generated output. The content lives in docs/protocols/modules/*.js and docs/cheatsheets/src/*.mjs. Change the module, then:

node docs/cheatsheets/build.mjs

node docs/cheatsheets/build.mjs --check reports whether any sheet has drifted from its protocol without rebuilding, which is worth running before a deploy. See docs/cheatsheets/AUTHORING.txt.