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Plate Pouring

Supervisor-only training

This technique is for supervisors only. Do not perform plate pouring unless you are a supervisor and have been trained to do it.

Required prerequisite training

Before doing this training, complete Flame Sterilization.

If you will also be remelting LB-agar in the microwave, complete Microwave.

Instructions

Use the plate pouring protocol for the detailed steps on preparing and pouring plates:

Plate pouring protocol

That protocol contains the step-by-step instructions for making and pouring plates.

The protocol builder at that link also includes protocols for other parts of this workflow, including remelting agar in the microwave, preparing antibiotics, and related steps.