Plate Pouring¶
Supervisor-only training¶
This technique is for supervisors only. Do not perform plate pouring unless you are a supervisor and have been trained to do it.
Required prerequisite training¶
Before doing this training, complete Flame Sterilization.
If you will also be remelting LB-agar in the microwave, complete Microwave.
Instructions¶
Use the plate pouring protocol for the detailed steps on preparing and pouring plates:
That protocol contains the step-by-step instructions for making and pouring plates.
The rest of the workflow (LB agar, 2YT, antibiotic stocks, sealing plates) is on Media and Plates, with a protocol and a video for each step.